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| Registros recuperados : 20 | |
| 15. |  | SÁ, F. P. De; PORTES, D. C.; LAZZAROTTO, M.; HELM, C. V.; WENDLING, I.; ZUFFELLATO-RIBAS, K. C. Teores de carboidratos em ministecas de erva mate. In:SEMANA DE ENGENHARIA DE ALIMENTOS DA UEPG, 17., 2016, Ponta Grossa. Anais. Ponta Grossa: UEPG, 2016. p. 63-67.| Biblioteca(s): Embrapa Florestas. |
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| 16. | | FRAGOSO, R. de O.; STUEPP, C. A.; SÁ, F. P. de; KRATZ, D.; ZUFFELLATO-RIBAS, K. C.; WENDLING, I. Vegetative rescue and ex vitro system production of Tibouchina sellowiana clonal plants by cutting and mini-cutting. Ciência Rural, Santa Maria, v.47, n. 11, e20160098, Nov. 2017.| Biblioteca(s): Embrapa Florestas. |
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| 17. | | SÁ, F. P. de; GOMES, E. N.; MAGGIONI, R. de A.; WENDLING, I.; HELM, C. V.; SANT'ANNA-SANTOS, B. F.; ZUFELLATO-RIBAS, K. C. Biochemical and anatomical features of adventitious rhizogenesis in apical and basal mini-cuttings of Ilex paraguariensis. New Forests, v. 53, p. 411-430, 2022.| Biblioteca(s): Embrapa Florestas. |
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| 18. | | LIMA, G. G. de; MATOS, M.; SÁ, F. P. de; MASHIBA, L. N.; MAGALHAES, W. L. E.; RACHWAL, M. F. G.; ZANATTA, J. A. Supraparticles as slow-release fertiliser in seedling potential growth of Eucalyptus urograndis and greenhouse gas fux impacts. Environmental Science and Pollution Research, v. 30, p. 23047-23059, 2023.| Biblioteca(s): Embrapa Florestas. |
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| 19. | | SÁ, F. P. de; BELNIAKI, A. C.; PANOBIANCO, M.; GABIRA, M. M.; KRATZ, D.; LIMA, E. A. de; WENDLING, I.; MAGALHAES, W. L. E. Peach palm residue compost as substrate for Bactris gasipaes self-sustaining seedlings production. International Journal of Recycling of Organic Waste in Agriculture, v. 9, n. 2, p. 183-192, 2020.| Biblioteca(s): Embrapa Florestas. |
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| 20. | | VIEIRA, L. M.; MAGGIONI, R. de A.; TOMASI, J. de C.; DUARTE, M. M.; AGUIAR, N. S. de; GABIRA, M. M.; SÁ, F. P. de; HELM, C. V.; WENDLING, I.; ZUFFELLATO-RIBAS, K. Miniestaquia e teores de compostos fenólicos de diferentes genótipos de erva-mate. In: EVENTOS ARAUCÁRIA: PESQUISA, INOVAÇÃO E TECNOLOGIAS PARA SISTEMAS DE PRODUÇÃO, ERVA-MATE XXI: INOVAÇÃO E TECNOLOGIAS PARA O SETOR ERVATEIRO, 2020, Colombo. Anais. Colombo: Embrapa Florestas, 2021. p. 46. (Embrapa Florestas. Documentos, 344). Resumo.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 20 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
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Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
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Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
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Palavras-Chave: |
Fertilização; Produção in vitro. |
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Thesagro: |
Bovino; Embrião; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500
001 2142749
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI
100 1 $aAMARAL, T. F.
245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource]
260 $c2022
520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer.
650 $aAnimal reproduction
650 $aEmbryo (animal)
650 $aIn vitro fertilization
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aFertilização
653 $aProdução in vitro
700 1 $aGRAZIA, J. G. V. de
700 1 $aMARTINHAO, L A. G.
700 1 $aDE COL, F.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aVIANA, J. H. M.
700 1 $aHANSEN, P. J.
773 $tScientific Reports$gv. 12, 7503, 2022.
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